Action Potential Recordings
The action potential is the consequence of the movements of ions through the different transmembrane ionic channels.
Therefore, recording cardiac action potentials, using the microelectrode technique, allows to investigate the repercussion of mix ion channels effects on cardiac electrical activity during early cardiac safety assessment.
The action potential assay sensitivity differs between species and cardiac tissues, depending on the ion channels blocked by the compound. Among species and cardiac tissues, action potential recordings in female rabbit Purkinje fibres constitute the most sensitive model to detect the effects of hERG blocking agents on cardiac repolarization.