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Pap­il­lary Muscles

Action poten­tial record­ings from guinea pig pap­il­lary muscles

Stan­dard Pro­to­col for GLP stud­ies:
  • six prepa­ra­tions
  • Three increas­ing cumu­la­tive con­cen­tra­tions of assay compound
  • Washout period or pos­i­tive con­trol at the end of the experiment
  • Stim­u­la­tion rates: 1 Hz; 0.2 Hz and 2 Hz (reverse use dependency)
Mea­sured para­me­ters:
  • Rest­ing poten­tial (RP) (mV)
  • Max­i­mal rate of depo­lar­iza­tion (dV/​dtmax) (V/​s)
  • Action Poten­tial Ampli­tude (APA) (mV)
  • Action Poten­tial Dura­tion at 50%, 70% and 90% of repo­lar­iza­tion (ms) = APD50, APD70, APD90


figure papillary muscle