hERG assays on Human Embryonic Kidney (HEK) cells:
Regulatory study (ICH S7B)
IKr current inhibition has been shown to prolong the cardiac action potential, a phenomenon associated with increased risk of QT interval prolongation, who can lead to potentially fatal ventricular tachyarhythmia called Torsades de Pointe. Inhibition of hERG channel by pharmaceuticals is the primary cause of drug-induced QT prolongation. A large number of drugs have been withdrawn from the market or during late stage clinical trials due to these cardiotoxic effects, therefore it’s important to identify potential inhibitory effects early in drug discover.
Mutation LQT2: LONG QT
|Standard hERG assay protocol:
|Reference compounds or positive controls