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hKV1.5

hKV1.5 assays on Human Embry­onic Kid­ney (HEK) cells

The human KV1.5 potas­sium chan­nel encodes for the ultra­ra­pid com­po­nent of the delayed rec­ti­fier potas­sium chan­nel IKur. In the car­diac tis­sue, KV1.5 potas­sium chan­nels are only expressed in atria in which it rep­re­sents a major com­po­nent of the car­diac repo­lar­i­sa­tion of atrial action poten­tial. Thus KV1.5 rep­re­sents a major tar­get for the treat­ment of atrial fib­ril­la­tion, the most com­mon sus­tained arrhythmia.

Stim­u­la­tion pro­to­col:


Mea­sured para­me­ters:
  • Ampli­tude of the tail cur­rent upon repo­lar­iza­tion to +80mV (pA)
  • Ampli­tude of the base cur­rent at –80mV (pA)
  • Inhi­bi­tion of hKV1.5 tail cur­rent amplitude (%)
  • IC50 value
Ref­er­ence com­pounds or pos­i­tive con­trols

REF­ER­ENCE

COM­POUNDS

IC50
PSORA-​4 5.5 nM
DPO-​1 68.0 nM
KN-​93 0.6 µM

PhysioStim’s other mod­els to study atrial drugs induced effects:

  • Lan­gen­dorff per­fused heart : ECG recordings
  • Rab­bit atria: Action poten­tial record­ings and atrial con­duc­tion velocity