Human cardiomyocytes hCM hiPSC-CM cardiomyocytes Human

Human iPSC Cardiomyocytes

Human cardiomyocytes derived from iPS cells for Pharmacological studies

Human cardiomyocytes derived from induced pluripotent stem cells (hiPSC-CM) provide rapid, reproducible and sensitive predictions of pharmacological effect on human normal and diseased cardiac tissue. It is also an integrative system that could easily identify potential cardiotoxic, cardioprotective, anti-arrhythmogenic, pro-arrhythmogenic, chronotropic and inotropic effects of compounds. Because the model is high throughput it is relevant to investigate series of compounds that could support you with ranking selection of candidates based on human pharmacology.

Human iPSC Cardiomyocytes & Pharmacology

Human cardiomyocytes derived from iPS cells provide the best solution to study the repercussion on ion channels and contractility during drug discovery and preclinical studies. This human in vitro model is used to evaluate the electrophysiology and the contractility of cardiomyocytes (normal and diseased). Abnormal cardiac situations such as tachycardia, bradycardia, contractility depression or medication toxicity could be mimicked by using various tools.

What is the added value of such a study?

Threshold of investigated pharmacological concentration.
Leading to the determination of the safety range versus efficacious concentration
To analyze drugs effects on excitation and contraction coupling in human heart
Ranking your compounds

Technique

xCELLigence RTCA Cardio ECR platform (48 wells plate format)
iCell®Cardiomyocytes² (Fujifilm)
Simultaneous recording of Electric Field Potential (EFP) and Impedance signals on the entire plate:
-EFP= Reflecting electrical activity

-Impedance= Reflecting cell contraction
Short and Long term exposure (up to 72h)
Disease Models (Option)
-Brugada Syndrome

-Dilated Cardiomyopathy

-Hypertrophic Cardiomyopathy

-Catecholaminergic Polymorphic Ventricular Tachycardia

Measured Parameters

Cell viability: Cell Index
Electrophysiology (EFP)
-Firing rate

-FPD as predictor of QT duration

-Spike amplitude
Contraction (Impedance)

-Amplitude of contraction

-Beating rate

-Individual beat duration (IBD10, 50, 90)

-Proarrhythmic events (BRI)

Upon Request : Biomarkers assays

Main advantages

Human tissue
Adjustable to your needs, manage the concentration and replication of each compound in the 48 wells.
Technically robust
Highly informative
Strongly replicable