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Human
Translational
Platform

Vasoreactivity platform

Human vascular tissue is the most relevant preclinical model for predicting the secondary pharmacology (efficacy and safety) of pharmaceutical compounds on vascular hemodynamic (variation of blood pressure). 

PhysioStim offers the opportunity to test your compounds on human artery and vein from healthy or diseased donors (in close partnership with hospitals and clinics).

/ Benefits

Translational: better predictivity


Animal ethics 3Rs: reduce the use of animals


Avoid species differences


Clinical characteristics of donors:  healthy and non-healthy vascular samples
 

Human reactivity with vessels

PhysioStim provides a wide range of vascular functional assays using different anatomical arterial and venous vessels, from large conductance to resistance vessels

Illustration d'un corps humain
illustration d'un socle
Diversity of serotonin (5-HT) responses according to the studied human vascular bed. Effect of cumulative concentrations of 5-HT on human vein and arteries reactivity using isolated organ baths. Contraction is expressed in % of KCl 60 mM reference contraction. Human vein (saphenous) and arteries (iliac and renal) are isolated from two different donors respectively.

Human large vessels

serotonin-induced contraction

/ Human primary Smooth Muscle Cells

Human vascular smooth muscle cell contraction measurement

Human vascular smooth muscle cells (SMC) isolation and culture

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/ Human primary Smooth Muscle Cells

Human vascular smooth muscle cell contraction measurement

xCelligence Real Time Cell Analyzer (CardioECR, AGILENT)

graphique

/ Human primary Smooth Muscle Cells

Human vascular smooth cell contraction measurement

Effects of 5-HT on Cell Index (CI) of smooth muscle cells (SMC) isolated from human aorta.

Human Cardiomyocytes for cardiac drug discovery

Human cardiomyocytes derived from induced pluripotent stem cells (hiPSC-CM) provide rapid, reproducible and sensitive prediction of QT prolongation. Besides QT prolongation it is also an integrative system that could easily identify potential cardiotoxic effects of compounds (pro-arrhythmogenic, cytotoxic and contractility worsening).

Schema triple cercle

This model is relevant to investigate series of compounds that could support the ranking selection of candidates based on human pharmacology.

/ Human Cardiomyocytes assays with a three-in-one assay

  • Primary screening assays
  • Cardiotoxicity evaluation and inotropism
  • CiPA assays for proarrhythmic liabilities (prediction of QT prolongation)